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<strong>Figure 1 WB Validation of TET1 in Human Cell Lines </strong><br>Loading: 15 μg of lysate Antibodies: TET1 7733, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 2 WB Validation of TET1 in Mouse Tissues </strong><br>Loading: 15 μg of lysate Antibodies: TET1 7733 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation of TET1 in Rat Tissues</strong><br> Loading: 15 μg of lysate Antibodies: TET1 7733, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunofluorescence Validation of TET1 in HeLa Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling TET1 with 7733 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
<strong>Figure 5Immunofluorescence Validation of TET1 in Mouse Brain Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TET1 with 7733 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI antibody (blue).
<strong> Figure 6 Immunohistochemistry Validation of TET1 in Human Testis Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TET1 antibody (7733) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody (7733) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of TET1 in Rat Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-TET1 antibody (7733) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 WB Validation of TET1 in Human Cell Lines
Loading: 15 μg of lysate Antibodies: TET1 7733, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

TET1 Antibody

CATALOG NUMBER: 7733

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (88%)
Immunogen:
Anti-TET1 antibody (7733) was raised against an 18 amino acid peptide near the carboxy terminus of human TET1.
The immunogen is located within amino acids 2030 - 2080 of TET1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 1 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot and Immunohistochemistry in human, mouse and rat samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
TET1 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with TET2 and TET3.
Positive Control 1:
Cat. No. 1405 – Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
Predicted: 235 kDa
Observed: 235 kDa
Isoforms:
Human TET1 has one isoform (2136aa, 235kD). Mouse TET1 has one isoform (2007aa, 219kD) and Rat TET1 also has one isoform (2033aa, 223kD). 7733 can detect human, mouse and rat.
Purification:
TET1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TET1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TET1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TET1
Additional Names:
TET1 Antibody: LCX, CXXC6, bA119F7.1, KIAA1676, LCX, Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6
Protein Accession Number:
NP_085128
Protein Gi Number:
156139122
Ncbi Gene Id Number:
80312
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TET1, a member of the ten-eleven-translocation (TET) family of genes, was initially discovered as a fusion partner of MLL in acute myeloid leukemias containing the t(10;11)(q22;q23) (1). It is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine (2). TET1 expression is regulated during inflammation in both THP-1 and primary dendritic cells, and is a negative transcriptional regulator of IL-1beta following and inflammatory stimulus (3). Recent evidence has shown that TET1 is part of a signaling pathway that regulates breast cancer growth and metastasis (4).
Background Reference 1:
Lorsbach, et al. Leukemia 2003; 17:637-41.
Background Reference 2:
Tahilani , et al. Science 2009; 324:930-5.
Background Reference 3:
Neves-Costa, et al. Immunol. 2013; 54:264-70.
Background Reference 4:
Sun, et al. Sci. USA 2013; 110:9920-5.
Citation 1:
McLaughlin et al. Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo. J Biol Chem. 2016;291(17):9014-24PMID: 26917720

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7733

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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