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Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TET3 in human brain tissue with TET3 antibody at 2.5 μg/ml.
Immunofluorescence of TET3 in human brain tissue with TET3 antibody at 20 μg/mL.
Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TET3 Antibody

CATALOG NUMBER: 7013

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TET3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TET3.
The immunogen is located within amino acids 1520 - 1570 of TET3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TET3 antibody can be used for detection of TET3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of TET3 are known to exist. TET3 antibody is predicted to not cross react with other TET proteins.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
183 kDa
Purification:
TET3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TET3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TET3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TET3
Additional Names:
TET3 Antibody: hCG_40738, KIAA0401, Methylcytosine dioxygenase TET3
Protein Accession Number:
NP_001274420
Protein Gi Number:
149944516
Ncbi Gene Id Number:
200424
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TET3 Antibody: TET3, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine and is most abundantly expressed in hematopoietic cells. Unlike the related TET2 protein, mutations in TET3 have not been observed in any myeloid malignancies. TET3 has been shown to be involved in the demethylation of zygotic DNA before the first mitosis and has been suggested to be involved in the epigenetic reprogramming of the zygotic paternal DNA following natural fertilization and may also contribute to somatic cell nuclear reprogramming during animal cloning.
Background Reference 1:
Langemeijer SM, Kuiper RP, Berends M, et al. Acquired mutations in TET2 are common in myelodysplastic syndromes. Nat. Genet. 2009; 41:838-42.
Background Reference 2:
Schaub FX, Looser R, Li S, et al. Clonal analysis of TET2 and JAK2 mutations suggests that TET2 can be a late event in the progression of myeloproliferative neoplasms. Blood 2011; 115:2003-7.
Background Reference 3:
Langemeijer SMC, Aslanyan MG, and Jansen JH. TET proteins in malignant hematopoiesis. Cell Cycle 2009; 4044-8.
Background Reference 4:
Gu TP, Guo F, Yang H, et al. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 2011; 477:606-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7013

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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