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Western blot analysis of extracts of various cell lines, using TFAM antibody (15-064) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse spleen using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SET antibody (14-466) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using TFAM antibody (15-064) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

TFAM Antibody

CATALOG NUMBER: 15-064

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
SW620
Positive Control 2:
HT-29
Predicted Molecular Weight:
Observed: 24kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TFAM
Additional Names:
Transcription Factor AntibodiesAM, TCF6, MTTF1, MTTFA, TCF6L1, TCF6L2, TCF6L3
Ncbi Gene Id Number:
7019
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-064

List Size:
100 uL

List Price:

$436.00

Datasheet
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