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Antibody used in WB on Mouse NIH-3T3 at 0.2-1 ug/ml.
Antibody used in WB on Mouse NIH-3T3 at 0.2-1 ug/ml.

TFAM Antibody

CATALOG NUMBER: 28-597

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse TFAM.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TFAM antibody can be used for detection of TFAM by ELISA at 1:62500. TFAM antibody can be used for detection of TFAM by western blot at 0.06 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight:
27 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TFAM antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TFAM
Additional Names:
TFAM, Hmgts, mtTFA, tsHMG, AI661103
Protein Accession Number:
NP_033386
Protein Gi Number:
6678303
Ncbi Gene Id Number:
21780
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TFAM a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this protein is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns Sayre syndrome was produced when expression of the gene was eliminated by targeted disruption in heart and muscle cells.
Background Reference 1:
Noack, H., et al., (2006) Biochim. Biophys. Acta 1760 (2), 141-150.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-597

List Size:
100 ul

List Price:

$519.00

Datasheet
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