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Western blot analysis of lysates from Hela, HepG2, U-2OS cell line (from left to right), using TFAM Antibody at 1:1000 at each lane.
Western blot analysis of lysate from 293T cell line, using TFAM Antibody at 1:1000.
TFAM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody . NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TFAM immunoreactivity is localized to mitochondrion significantly.
Western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).
Western blot analysis of lysates from Hela, HepG2, U-2OS cell line (from left to right), using TFAM Antibody at 1:1000 at each lane.

TFAM Antibody

CATALOG NUMBER: 55-117

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TFAM
Additional Names:
Transcription factor A, mitochondrial, mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM, TCF6, TCF6L2
Protein Accession Number:
Q00059
Protein Gi Number:
417324
Ncbi Gene Id Number:
7019
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a mitochondrial transcription factorthat is a key activator of mitochondrial transcription as well as aparticipant in mitochondrial genome replication. Studies in micehave demonstrated that this gene product is required to regulatethe mitochondrial genome copy number and is essential for embryonicdevelopment. A mouse model for Kearns-Sayre syndrome was producedwhen expression of this gene was eliminated by targeted disruptionin heart and muscle cells.
Background Reference 1:
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Background Reference 2:
Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010)
Background Reference 3:
Akhmetov, I.I., et al. Fiziol Cheloveka 36(2):121-125(2010)
Background Reference 4:
Shulman, J.M., et al. PLoS ONE(6), E11244 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-117

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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