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Western blot analysis in Hela,HL-60 cell line lysates (35ug/lane).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela,HL-60 cell line lysates (35ug/lane).

TFAP4 Antibody

CATALOG NUMBER: 56-148

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human TFAP4.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TFAP4
Additional Names:
Transcription factor AP-4, Activating enhancer-binding protein 4, Class C basic helix-loop-helix protein 41, bHLHc41, TFAP4, BHLHC41
Protein Accession Number:
Q01664
Protein Gi Number:
1729833
Ncbi Gene Id Number:
7023
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Transcription factors of the basic helix-loop-helix-zipper(bHLH-ZIP) family contain a basic domain, which is used for DNAbinding, and HLH and ZIP domains, which are used foroligomerization. Transcription factor AP4 activates both viral andcellular genes by binding to the symmetrical DNA sequence CAGCTG(Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed2123466]).
Background Reference 1:
Ku, W.C., et al. Mol. Cell Proteomics 8(9):2034-2050(2009)
Background Reference 2:
Cao, J., et al. Int. J. Surg. Pathol. 17(1):16-21(2009)
Background Reference 3:
Jung, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(39):15046-15051(2008)
Background Reference 4:
Pons, J., et al. Biochemistry 47(1):14-29(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-148

List Size:
400 ul

List Price:

$495.00

Datasheet
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