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Antibody used in IHC on Human Intestine.
Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Antibody used in IHC on Human Intestine.

TFB2M Antibody

CATALOG NUMBER: 28-102

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TFB2M.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
TFB2M antibody can be used for detection of TFB2M by ELISA at 1:312500. TFB2M antibody can be used for detection of TFB2M by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
45 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TFB2M antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TFB2M
Additional Names:
TFB2M, FLJ22661, FLJ23182, Hkp1, mtTFB2
Protein Accession Number:
NP_071761
Protein Gi Number:
11641289
Ncbi Gene Id Number:
64216
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TFB2M is a S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. It is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. It stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
Background Reference 1:
Falkenberg, M., (2002) Nat. Genet. 31 (3), 289-294.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-102

List Size:
100 ul

List Price:

$473.00

Datasheet
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