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45-159 (1.0ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-159 (0.5ug/ml) staining of HeLa (A) and (0.3ug/ml) Jurkat (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-159 (1.0ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TFEB Antibody

CATALOG NUMBER: 45-159

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: ASRIGLRMQLMRE-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55kDa band observed in Human Thymus and in lysates of cell line Jurkat and approx. 60kDa in lysates of cell line HeLa, (calculated MW of 52.9kDa according to NP_009093.1 and 54.2kDa according to NP_001161299.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on cell line NIH3T3 for this particular batch.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TFEB
Additional Names:
TFEB, transcription factor EB, TCFEB, T-cell transcription factor EB, RP4-696P19.3, AlphaTFEB, TCFEB, bHLHe35, OTTHUMP00000043540,
Protein Accession Number:
NP_009093.1,NP_001161299.2,NP_001258872.1
Protein Gi Number:
24307933
Ncbi Gene Id Number:
7942
Background Reference 1:
Steingrimsson E, Sawadogo M, Gilbert DJ, Zervos AS, Brent R, Blanar MA, Fisher DE, Copeland NG, Jenkins NA. Murine chromosomal location of five bHLH-Zip transcription factor genes. Genomics. 1995 Jul 20;28(2):179-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-159

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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