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Western blot analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB (NT) antibody at 2.5 μg/mL.
Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 μg/mL.
Western blot analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TFEB Antibody

CATALOG NUMBER: 6801

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
TFEB antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TFEB.
The immunogen is located within amino acids 70 - 120 of TFEB.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TFEB antibody can be used for detection of TFEB by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Purification:
TFEB Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TFEB Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TFEB antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TFEB
Additional Names:
TFEB Antibody: TCFEB, BHLHE35, ALPHATFEB, Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35
Protein Accession Number:
NP_009093
Protein Gi Number:
24307933
Ncbi Gene Id Number:
7942
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TFEB Antibody: The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.
Background Reference 1:
Carr CS and Sharp PA. A helix-loop-helix protein related to the immunoglobulin E box-binding proteins. Mol. Cell Biol. 1990; 10:4384-8.
Background Reference 2:
Davis IJ, Hsi BL, Arroyo JD, et al. Cloning of an alpha-TFEB fusion in renal tumors harboring the t(6;11)(p21;q13) chromosome translocation. Proc. Natl. Acad. Sci. USA 2003; 100:6051-6.
Background Reference 3:
Sardiello M, Palmieri M, di Ronza A, et al. A gene network regulating lysosomal biogenesis and function. Science 2009; 325:473-7.
Background Reference 4:
Pena-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, et al. Regulation of TFEB and V-ATPases my mTORC1. EMBO J. 2011; 30:3242-58

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6801

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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