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43-490 (1ug/ml) staining of Colon cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-490 (1ug/ml) staining of Colon cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TFF3 Antibody

CATALOG NUMBER: 43-490

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: SANQCAVPAKDRVD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 13kDa band observed in Human Colon cancer, and in normal Duodenum and Ileum lysates (calculated MW of 10.2kDa according to NP_003217.3). Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 13kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TFF3
Additional Names:
TFF3, trefoil factor 3 (intestinal), ITF, P1B, TFI, polypeptide P1.B, trefoil factor 3
Protein Accession Number:
NP_003217.3
Protein Gi Number:
119629977
Ncbi Gene Id Number:
7033
Background Reference 1:
Raja SB, Murali MR, Devaraj H, Devaraj SN.Differential expression of gastric MUC5AC in colonic epithelial cells: TFF3-wired IL1 β/Akt crosstalk-induced mucosal immune response against Shigella dysenteriae infection.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-490

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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