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Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 μg/mL.
Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 μg/mL.
Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 μg/ml.
Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 μg/ml. <br><br>Red: TFF3 Antibody (6113) <br> Blue: DAPI staining
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2 μg/ml.
Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Green Product
Trial Size

TFF3 Antibody

CATALOG NUMBER: 6113

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (81%), Pig: (81%)
Immunogen:
TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3.
The immunogen is located within the last 50 amino acids of TFF3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry at starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
TFF3 antibody is predicted to not cross-react with other TFF family members.
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 10 kDa
Observed: 11 kDa
Purification:
TFF3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
TFF3 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TFF3 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TFF3
Additional Names:
TFF3 Antibody: ITF, P1B, TFI, ITF, Trefoil factor 3, Intestinal trefoil factor, hITF
Protein Accession Number:
NP_003217
Protein Gi Number:
119629977
Ncbi Gene Id Number:
7033
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TFF3 Antibody: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.
Background Reference 1:
Podolsky DK, Lynch-Devaney K, Stow JL, et al. Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion. J. Biol. Chem. 1993; 268:6694-702.
Background Reference 2:
Gott P, Beck S, Machado JC, et al. Human trefoil peptides: genomic structure in 21q22.3 and coordinated expression. Eur. J. Hum. Genet. 1996; 4:308-15.
Background Reference 3:
Hernandez C, Santamatilde E, McCreath KJ, et al. Induction of trefoil factor TFF1, TFF2 and TFF3 by hypoxia is mediated by hypoxia inducible factor-1: implications for gastric mucosal healing. Br. J. Pharmacol. 2009; 156:262-72.
Background Reference 4:
Thim L. Trefoil peptides: from structure to function. Cell Mol. Life Sci. 1997; 53:888-903.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6113

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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