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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).

TFIIIC90 Antibody

CATALOG NUMBER: 56-142

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This TFIIIC90 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-244 amino acids from the N-terminal region of human TFIIIC90.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
92 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GTF3C4
Additional Names:
General transcription factor 3C polypeptide 4, TF3C-delta, Transcription factor IIIC 90 kDa subunit, TFIIIC 90 kDa subunit, TFIIIC90, Transcription factor IIIC subunit delta, GTF3C4
Protein Accession Number:
Q9UKN8
Protein Gi Number:
212276467
Ncbi Gene Id Number:
9329
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Background Reference 1:
Joubert, B.R., et al. Genome Med(3), 17 (2010) :
Background Reference 2:
Dumay-Odelot, H., et al. J. Biol. Chem. 282(23):17179-17189(2007)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 4:
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-142

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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