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45-160 staining (0.3ug/ml) of Hela lysate (RIPA buffer, 35ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing TFPI and probed with 45-160 (mock transfection in first lane).
45-160 staining (0.3ug/ml) of Hela lysate (RIPA buffer, 35ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TFPI Antibody

CATALOG NUMBER: 45-160

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-VKIAYEEIFVKNM
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38kDa to 45kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize isoform a (NP_006278.1) only.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TFPI
Additional Names:
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor), TFPI, EPI, LACI, TFI, TFPI1, OTTHUMP00000163472, OTTHUMP00000205431, anti-convertin, extrinsic pathway inhibitor, lipoprotein-associated coagulation inhibitor, tissue factor pathway inhibitor
Protein Accession Number:
NP_006278.1
Protein Gi Number:
5454114
Ncbi Gene Id Number:
7035
Background Reference 1:
Wun TC, Kretzmer KK, Girard TJ, Miletich JP, Broze GJ Jr. Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains. J Biol Chem 1988 May 5;263(13):6001-4

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-160

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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