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Western blot analysis using TGF beta1 monoclonal antibody against truncated TGF beta1 recombinant protein.
Western blot analysis using TGF beta1 monoclonal antibody against truncated TGF beta1 recombinant protein.

TGFbeta1 Antibody [4F9C10]

CATALOG NUMBER: 32-224

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant TGFbeta1 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG2a
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
TGF beta1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TGFB1
Additional Names:
TGFBTGF-beta-1, CED, LAP, DPD1, TGFB, TGFbeta
Protein Accession Number:
P01137
Protein Gi Number:
135674
Ncbi Gene Id Number:
7040
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TGF beta(transforming growth factor beta), with 390-amino acid protein (about 43 kDa), is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. In mammals, three isoforms of TGFbeta, that is, beta1, beta2,and beta3,are known. TGF beta is one of numerous inhibitory factors produced by cancer cells that regulate antitumor immunity. TGF beta1 takes part in the local response in the course of primary lung cancer and TGFbeta1 is thought to be implicated in breast cancer progression.TGFbeta1 also plays a critical role in the downregulation of microglial responses minimizing brain inflammation and thus avoiding exacerbation of brain damage.
Background Reference 1:
Delphine Boche, Colm Cunningham, Fabian Docagne. Neurobiol Dis. 2006 Feb 27
Background Reference 2:
Shizuya Saika. Lab Invest. 2006 Feb;86(2):106-15

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-224

List Size:
0.1 mg

List Price:

$345.00

Datasheet
Shipping Info

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