Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of TGFBI using rabbit polyclonal TGFBI Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGFBI gene.

TGFBI Antibody

CATALOG NUMBER: 58-664

Clonality:

Polyclonal

Tested Applications:

Flow, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Homology:

Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rabbit

Immunogen:

This TGFBI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human TGFBI.

Conjugate:

Unconjugated

Tested Applications:

Flow, WB

Application Note:

For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

75 kDa

Advanced Validation

Properties

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

TGFBI

Additional Names:

Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP, TGFBI, BIGH3

Protein Accession Number:

Q15582

Protein Gi Number:

2498193

Ncbi Gene Id Number:

7045

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene encodes an RGD-containing protein that binds totype I, II and IV collagens. The RGD motif is found in manyextracellular matrix proteins modulating cell adhesion and servesas a ligand recognition sequence for several integrins. Thisprotein plays a role in cell-collagen interactions and may beinvolved in endochondrial bone formation in cartilage. The proteinis induced by transforming growth factor-beta and acts to inhibitcell adhesion. Mutations in this gene are associated with multipletypes of corneal dystrophy.

Background Reference 1:

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)

Background Reference 2:

Edelstein, S.L., et al. Cornea 29(6):698-700(2010)

Background Reference 3:

Romero, P., et al. Mol. Vis. 16, 1601-1609 (2010) :

Background Reference 4:

Paliwal, P., et al. Mol. Vis. 16, 1429-1438 (2010) :

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-664

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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TGFBI Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

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