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Western blot analysis of lysate from A549 cell line, using TGIF1 Antibody at 1:1000.
Western blot analysis of lysate from A549 cell line, using TGIF1 Antibody at 1:1000.

TGIF1 Antibody

CATALOG NUMBER: 57-827

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TGIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-109 amino acids from the N-terminal region of human TGIF1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TGIF1
Additional Names:
Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1, TGIF1, TGIF
Protein Accession Number:
Q15583
Protein Gi Number:
215274200
Ncbi Gene Id Number:
7050
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thethree-amino acid loop extension (TALE) superclass of atypicalhomeodomains. TALE homeobox proteins are highly conservedtranscription regulators. This particular homeodomain binds to apreviously characterized retinoid X receptor responsive elementfrom the cellular retinol-binding protein II promoter. In additionto its role in inhibiting 9-cis-retinoic acid-dependent RXR alphatranscription activation of the retinoic acid responsive element,the protein is an active transcriptional co-repressor of SMAD2 andmay participate in the transmission of nuclear signals duringdevelopment and in the adult. Mutations in this gene are associatedwith holoprosencephaly type 4, which is a structural anomaly of thebrain. Alternative splicing has been observed at this locus andeight variants, encoding four distinct isoforms, are described.
Background Reference 1:
Bengoechea-Alonso, M.T., et al. Oncogene 29(38):5322-5328(2010)
Background Reference 2:
Paulussen, A.D., et al. Eur. J. Hum. Genet. 18(9):999-1005(2010)
Background Reference 3:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 4:
Demange, C., et al. Mol. Cell 36(6):1073-1085(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-827

List Size:
400 ul

List Price:

$495.00

Datasheet
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