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46-484 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-484 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TGIF2 Antibody

CATALOG NUMBER: 46-484

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-EMELQKQQDP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 22kDa band observed in lysates of A431, A549 and HEK293 cell lines (calculated MW of 25.9kDa according to NP_068581.1). Recommended concentration: 0.3-1.0ug/ml.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TGIF2
Additional Names:
TGIF2, TGFB-induced factor 2 (TALE family homeobox), homeobox protein TGIF2, 5'-TG-3' interacting factor 2, TGF(beta)-induced transcription factor 2, transcription growth factor-beta-induced factor 2, TGFB-induced factor homeobox 2, OTTHUMP00000030849
Protein Accession Number:
NP_068581.1
Protein Gi Number:
11141881
Ncbi Gene Id Number:
60436
Background Reference 1:
Melhuish TA, Gallo CM, Wotton D. TGIF2 interacts with histone deacetylase 1 and represses transcription. J Biol Chem. 2001 Aug 24;276(34):32109-14. Epub 2001 Jun 26.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-484

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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