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Western blot analysis of extracts of various cell lines, using TGM1 antibody (14-944) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using TGM1 antibody (14-944) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat skin cells using TGM1 antibody (14-944) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse skin cells using TGM1 antibody (14-944) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using TGM1 antibody (14-944) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

TGM1 Antibody

CATALOG NUMBER: 14-944

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human TGM1 (NP_000350.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
Mouse kidney
Positive Control 3:
Rat kidney
Positive Control 4:
Rat brain
Predicted Molecular Weight:
Observed: 90kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TGM1
Additional Names:
TGM1, ICR2, KTG, LI, LI1, TGASE, TGK, ARCI1
Ncbi Gene Id Number:
7051
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-944

List Size:
100 uL

List Price:

$436.00

Datasheet
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