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Western blot analysis of TGM4 Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostate carcinoma with TGM4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells using TGM4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TGM4 Antibody in CEM cell line lysates (35ug/lane)

TGM4 Antibody

CATALOG NUMBER: 62-536

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TGM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human TGM4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
77 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TGM4
Additional Names:
Protein-glutamine gamma-glutamyltransferase 4, Fibrinoligase, Prostate transglutaminase, Prostate-specific transglutaminase, Transglutaminase P, TG(P), TGP, TGase P, Transglutaminase-4, TGase-4, TGM4
Protein Accession Number:
P49221
Protein Gi Number:
3915892
Ncbi Gene Id Number:
7047
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TGM4is associated with the mammalian reproductive process and catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.
Background Reference 1:
Jiang,W.G., et.al., Microvasc. Res. 77 (2), 150-157 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-536

List Size:
400 ul

List Price:

$495.00

Datasheet
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