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Western blot analysis of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of THEM2 in human liver tissue with THEM2 antibody at 2.5 μg/mL.
Immunofluorescence of THEM2 in human liver tissue with THEM2 antibody at 20 μg/mL.
Western blot analysis of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

THEM2 Antibody

CATALOG NUMBER: 5257

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
THEM2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human THEM2.
The immunogen is located within the last 50 amino acids of THEM2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
THEM2 antibody can be used for detection of THEM2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
THEM2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
THEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
THEM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACOT13
Additional Names:
THEM2 Antibody: HT012, THEM2, PNAS-27, HT012, Acyl-coenzyme A thioesterase 13, Thioesterase superfamily member 2, Acyl-CoA thioesterase 13
Protein Accession Number:
NP_060943
Protein Gi Number:
8923812
Ncbi Gene Id Number:
55856
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
THEM2 Antibody: THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several tissues such as heart, kidney, liver and brain and has been shown to be co-localized with beta-tubulin on microtubules. THEM2 interacts with StarD, a protein that plays a key role in fatty acid metabolism, and the addition of StarD to THEM2 increased its catalytic activity, suggesting that THEM2 plays a significant role in the metabolism of fatty acids. At least two isoforms of THEM2 are known to exist.
Background Reference 1:
Cheng Z, Song F, Shan X, et al. Crystal structure of human thioesterase superfamily member 2. Biochem. Biophys. Res. Commun.2006; 349:172-7.
Background Reference 2:
Wei J, Kang HW, and Cohen DE. Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs. Biochem. J.2009; 421:311-22.
Background Reference 3:
Cheng Z, Bao S, Shan X, et al. Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule. Biochem. Biophys. Res. Commun.2006; 350:850-3.
Background Reference 4:
Scappa EF, Pocai A, Wu MK, et al. Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD. FASEB J.2008; 22:2579-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5257

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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