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Western blot analysis of THEM4 in human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of THEM4 in human liver tissue with THEM4 antibody at 2.5 μg/mL.
Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 μg/mL.
Western blot analysis of THEM4 in human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

THEM4 Antibody

CATALOG NUMBER: 5227

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4.
The immunogen is located within amino acids 60 - 110 of THEM4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
THEM4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
THEM4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
THEM4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
THEM4
Additional Names:
THEM4 Antibody: CTMP, CTMP, Acyl-coenzyme A thioesterase THEM4, Carboxyl-terminal modulator protein, Acyl-CoA thioesterase THEM4
Protein Accession Number:
NP_444283
Protein Gi Number:
76159293
Ncbi Gene Id Number:
117145
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
THEM4 Antibody: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.
Background Reference 1:
Maira SM, Galetic I, Brazil DP, et al. Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane. Science2001; 294:374-80.
Background Reference 2:
Knobbe CB, Reifberger J, Blaschke B, et al. Hypermethylation and transcriptional downregulation of the carboxyl-terminal modulator protein gene in glioblastomas. J. Natl. Cancer Inst.2004; 96:483-6.
Background Reference 3:
Zhao H, Martin BM, Bisoffi M, et al. The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the hotdog-fold family. Biochemistry2009; 48:5507-9.
Background Reference 4:
Parcellier A, Tintignac LA, Zhuravleva E, et al. Carboxy-terminal modulator protein (CTMP) is a mitochondrial protein that sensitizes cells to apoptosis. Cell Signal.2009; 21:639-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5227

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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