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Western blot analysis using Trx monoclonal antibody against four different fusion protein with truncated Trx tag.
Western blot analysis using Trx monoclonal antibody against four different fusion protein with truncated Trx tag.

Thioredoxin Antibody [1H6H6]

CATALOG NUMBER: 32-225

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human Thioredoxin (TRX).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Dilution
Western Blot:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Thioredoxin (TRX) monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLRB1
Additional Names:
CLEC5B, NKRP1A, Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, NKR-P1A, NKR, CD161, NKR-P1, hNKR-P1A
Protein Accession Number:
Q12918
Protein Gi Number:
74722301
Ncbi Gene Id Number:
3820
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Thioredoxin (TRX) is a small ubiquitous protein (MW12 kDa) which is exist in a wide variety of prokaryotic and eukaryotic cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site.This antibody is suitable for detecting fusion proteins which encode a Trx-Tag by immunoblotting and immunoprecipitation.The Monoclonal Antibody can detect a little Trx-Tag fusion proteins with negligible cross-reactivity with bacterial, insect, or mammalian lysates.
Background Reference 1:
Holmgen, A. et al., Annu. Rev. Biochem. 54, 237-271 (1985).
Background Reference 2:
Wollman, E. E. et al., J. Biol. Chem. 263, 15506-15512 (1988).
Background Reference 3:
Sasada, T. et al., J. Toxicol. Sci. 21, 285-287 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-225

List Size:
0.1 mL

List Price:

$345.00

Datasheet
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