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43-222 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-222 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

THNSL2 Antibody

CATALOG NUMBER: 43-222

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SRFRHREVVHLSR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Colorectal Cancer lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.0kDa according to NP_001231605.1 and 54.1kDa according to NP_060741.3. The 35kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_060741.3) and isoform 2 (NP_001231605.1) only.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
THNSL2
Additional Names:
THNSL2, threonine synthase-like 2 (S. cerevisiae), FLJ10916, FLJ35504, SOFAT, THS2, TSH2, OTTHUMP00000160830, OTTHUMP00000160831, secreted osteoclastogenic factor of activated T cells, threonine synthase-like 2
Protein Accession Number:
NP_060741.3,NP_001231605.1
Protein Gi Number:
149193323
Ncbi Gene Id Number:
55258
Background Reference 1:
Rifas L, Weitzmann MN.A novel T cell cytokine, secreted osteoclastogenic factor of activated T cells, induces osteoclast formation in a RANKL-independent manner.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-222

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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