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46-487 (0.5ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-487 (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-487 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-487 (0.5ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

THRA Antibody

CATALOG NUMBER: 46-487

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KEEMIRSLQQRP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60+50kDa bands observed in Human Bone Marrow lysates (calculated MW of 54.8kDa according to NP_003241.2 and of 46.8kDa according to NP_955366.1). Recommended concentration: 0.5-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Breast. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_955366.1; NP_003241.2). This antibody is not expected to cross-react to THRB.
Positive Control 1:
Human Bone Marrow Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
THRA
Additional Names:
THRA, thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian), AR7, EAR7, ERB-T-1, ERBA, ERBA1, MGC000261, MGC43240, NR1A1, THRA1, THRA2, c-ERBA-1, ERBA-related 7, avian erythroblastic leukemia viral (v-erb-a) oncogene homolog, thyroid hormone receptor, alpha, triiodothyronine receptor
Protein Accession Number:
NP_955366.1,NP_003241.2
Protein Gi Number:
40806160
Ncbi Gene Id Number:
7067
Background Reference 1:
Nishiyama K, Baba S, Yamada T, Matsushita A, Natsume H, Nakano K, Sasaki S, Nakamura H. Embryonic lethal effect of expressing a dominant negative mutant human thyroid hormone receptor alpha1 in mice. Endocr J. 2003 Oct;50(5):561-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-487

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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