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IHC testing of FFPE human cervical carcinoma with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human bladder carcinoma with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human placenta with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of 1) recombinant partial protein and 2) human ThP1 lysate with Thrombomodulin antibody (clone THBD/1591). Expected molecular weight ~60/100 kDa (unmodified/glycosylated).
SDS-PAGE Analysis of Purified, BSA-Free Thrombomodulin Antibody (clone THBD/1591). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human cervical carcinoma with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Thrombomodulin Antibody [THBD/1591]

CATALOG NUMBER: 33-761

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 0.5-1 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
ELISA: 2-4 ug/ml; order the BSA-free formulation for coating
WB: 0.5-1 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
THBD
Ncbi Gene Id Number:
7056
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-761

List Size:
100 ug

List Price:

$626.00

Datasheet
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