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Western blot analysis of lysates from Recombinant Mouse Thrombopoietin/Tpo Protein (RP01665) using Thrombopoietin/Tpo Rabbit mAb (A26325) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 10ng,5ng μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 20s.
Western blot analysis of lysates from Recombinant Mouse Thrombopoietin/Tpo Protein (RP01665) using Thrombopoietin/Tpo Rabbit mAb (A26325) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 10ng,5ng μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 20s.

Thrombopoietin/Tpo Rabbit Recombinant Antibody

CATALOG NUMBER: 83-282

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:4000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Recombinant Mouse Thrombopoietin/Tpo Protein (RP01665)
Predicted Molecular Weight:
38kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Thpo
Additional Names:
Ml; Tpo; Mgdf; Mpllg
Protein Accession Number:
P40226
Ncbi Gene Id Number:
21832
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. The encoded protein is a ligand for the product of the myeloproliferative leukemia virus oncogene. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-282

List Size:
100 uL

List Price:

$477.00

Datasheet
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