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43-469 (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-469 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
43-469 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining. The nuclear
43-469 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol
43-469 (5ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
43-469 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
43-469 (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Thrombospondin Antibody

CATALOG NUMBER: 43-469

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: NRIPESGGDNSVFD-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Thrombospondin
Additional Names:
THBS1, thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180
Protein Accession Number:
NP_003237.2
Protein Gi Number:
40317626
Ncbi Gene Id Number:
7057
Background Reference 1:
Pan MR, Chang HC, Chuang LY, Hung WC.The nonsteroidal anti-inflammatory drug NS398 reactivates SPARC expression via promoter demethylation to attenuate invasiveness of lung cancer cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-469

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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