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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human THBS1 with C-terminal MYC tag probed with 43-470 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-470 (1ug/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human THBS1 with C-terminal MYC tag probed with 43-470 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-470 (1ug/ml) in Lane B. Primary

Thrombospondin Antibody

CATALOG NUMBER: 43-470

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: EGEARETKACKKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:In transfected HEK293 transiently expressing Human THBS1 a band of approx. 200kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 129kDa according to NP_003237.2. Recommended concentration, 1-3ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Thrombospondin
Additional Names:
thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180
Protein Accession Number:
NP_003237.2
Protein Gi Number:
40317626
Ncbi Gene Id Number:
7057
Background Reference 1:
Samols MA, Skalsky RL, Maldonado AM, Riva A, Lopez MC, Baker HV, Renne R.Identification of ular genes targeted by KSHV-encoded microRNAs.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-470

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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