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Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Thymidylate Synthase antibody (TS106).
SDS-PAGE Analysis of Purified, BSA-Free Thymidylate Synthase Antibody (clone TS106). Confirmation of Integrity and Purity of the Antibody.
Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Thymidylate Synthase antibody (TS106).

Thymidylate Synthase Antibody [TS106]

CATALOG NUMBER: 33-561

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human protein was used as the immunogen for this Thymidylate Synthase antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the Thymidylate Synthase antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
TYMS
Additional Names:
TYMS, HsT422, MGC88736, TMS, TS, TSase, HST422
Ncbi Gene Id Number:
7298
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-561

List Size:
100 ug

List Price:

$626.00

Datasheet
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