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Western blot analysis of Thymopoietin in HeLa cell lysate with Thymopoietin antibody at 0.25 μg/mLl in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of Thymopoietin in HeLa cells with Thymopoietin antibody at 2.5 μg/mL.
Immunofluorescence of Thymopoietin in HeLa cells with Thymopoietin antibody at 5 μg/mL.
Western blot analysis of Thymopoietin in HeLa cell lysate with Thymopoietin antibody at 0.25 μg/mLl in (A) the absence and (B) the presence of blocking peptide.
Trial Size

Thymopoietin Antibody

CATALOG NUMBER: 6605

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (95%)
Immunogen:
Thymopoietin antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human Thymopoietin.
The immunogen is located within amino acids 80 - 130 of Thymopoietin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Thymopoietin antibody can be used for detection of Thymopoietin by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of Thymopoietin are known to exist; this antibody will detect all isoforms. Thymopoietin antibody is predicted to not cross-react with other LEM domain-containing proteins.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
Thymopoietin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Thymopoietin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Thymopoietin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TMPO
Additional Names:
Thymopoietin Antibody: TP, LAP2, CMD1T, LEMD4, PRO0868, Lamina-associated polypeptide 2, isoform alpha, Thymopoietin isoform alpha, TP alpha
Protein Accession Number:
NP_003267
Protein Gi Number:
4507555
Ncbi Gene Id Number:
7112
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Thymopoietin Antibody: Thymopoietin (TMPO), also known as LAP2, is a widely expressed lem-domain-containing nuclear protein that is thought to play an important role in the regulation of nuclear architecture by binding lamin B1 and chromosomes in a manner regulated by phosphorylation during mitosis. Thymopoietin associates with nucleoplasmic lamins A/C, which are encoded by the dilated cardiomyopathy (DCM) gene LMNA; mutations in Thymopoietin disrupt this binding, suggesting that Thymopoietin may be a candidate disease gene for DCM.
Background Reference 1:
Harris CA, Andryuk PJ, Cline SW, et al. Structure and mapping of the human thymopoietin (TMPO) gene and relationship of human TMPO beta to rat lamin-associated polypeptide 2. Genomics 1995; 28:198-205.
Background Reference 2:
Taylor MR, Slavov D, Gajewski A, et al. Thymopoietin (lamina-associated polypeptide 2) gene mutation associated with dilated cardiomyopathy. Hum. Mutat. 2005; 26:566-

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6605

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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