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Western blot analysis of extracts of BT-474 cells, using TIA1 antibody (22-073) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using TIA1 Antibody (22-073) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TIA1 Antibody (22-073) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TIA1 antibody (22-073) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TIA1 antibody (22-073). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of BT-474 cells, using TIA1 antibody (22-073) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

TIA1 Antibody

CATALOG NUMBER: 22-073

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human TIA1 (NP_071505.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100
Positive Control 1:
BT-474
Predicted Molecular Weight:
Observed: 54kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TIA1
Additional Names:
TIA1, WDM, TIA-1
Ncbi Gene Id Number:
7072
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-073

List Size:
50 uL

List Price:

$295.00

Datasheet
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