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Western blot analysis of TIAF in K562 cell lysate with TIAF antibody at 1 μg/mL.
Immunocytochemistry of TIAF in K562 cells with TIAF antibody at 2.5 μg/ml.
Western blot analysis of TIAF in K562 cell lysate with TIAF antibody at 1 μg/mL.
Trial Size

TIAF Antibody

CATALOG NUMBER: 7305

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TIAF antibody was raised against a 16 amino acid peptide near the amino terminus of human TIAF .
The immunogen is located within the first 50 amino acids of TIAF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
TIAF Antibody can be used for detection of TIAF by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
Two alternatively spliced transcript variants encoding different isoforms have been identified.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Predicted Molecular Weight:
Predicted: 13 kDa
Observed: 14 kDa
Purification:
TIAF Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TIAF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TIAF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TIAF
Additional Names:
TIAF Antibody: MAJN, SPR210, TGFB1-induced anti-apoptotic factor 1, 12 kDa TGF-beta-1-induced antiapoptotic factor
Protein Accession Number:
NP_004731
Protein Gi Number:
17978509
Ncbi Gene Id Number:
9220
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TIAF Antibody: TIAF-1 (TGFB1-induced anti-apoptotic factor 1), also known as MYO18A, is a TGFB1-induced anti-apoptotic factor that functions to inhibit the cytotoxic effects of TNF-alpha, TRADD and FADD. It localizes to the nucleus and significantly up-regulated in activated helper T lymphocytes (TH2) in chronic kidney and liver allograft rejection. Suppression of TIAF1 expression by siRNA prevents UV irradiation-mediated p53 phosphorylation and nuclear translocation. TIAF1 is essential in the p53- and WOX1-mediated cell death and shows an aggregation-dependent control of tumor progression and metastasis, and regulation of cell death.
Background Reference 1:
Chang NS, Mattison J, Cao H, et al. Cloning and characterization of a novel transforming growth factor-beta1-induced TIAF1 protein that inhibits tumor necrosis factor cytotoxicity. Biochem. Biophys. Res. Commun. 1998; 253:743-9.
Background Reference 2:
Khera S and Chang NS. TIAF1 participates in the transforming growth factor beta1--mediated growth regulation. Ann. N.Y. Acad. Sci. 2003; 995:11-21.
Background Reference 3:
Van der Leij J, Van den Berg A, Albrecht EW, et al. High expression of TIAF-1 in chronic kidney and liver allograft rejection and in activated T-helper cells. Transplantation 2003; 75:2076-82.
Background Reference 4:
Schultz L, Khera S, Sleve D, et al. TIAF1 and p53 functionally interact in mediating apoptosis and silencing of TIAF1 abolishes nuclear translocation of serine 15-phosphorylated p53. DNA Cell Biol. 2004; 23:67-74

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7305

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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