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Western blot analysis of extract from JK, T98G, COS7 and HeLa cells using TIF1β Antibody.
Western blot analysis of extract from JK, T98G, COS7 and HeLa cells using TIF1β Antibody.

TIF1beta Antibody

CATALOG NUMBER: 79-736

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TIF1beta antibody was raised against a peptide sequence around aa.827~831 (L-S-G-G-P) derived from Human TIF1β.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total TIF1β protein.
Predicted Molecular Weight:
110 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
TRIM28
Additional Names:
KAP1, TF1B, RNF96, TIF1B, KAP1
Protein Accession Number:
NP_005753.1
Protein Gi Number:
5032179
Ncbi Gene Id Number:
10155
Background:
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.
Background Reference 1:
Friedman J. R., Fredericks W. J., Jensen D. E., Speicher D. W., Huang X. -P., Neilson E. G., Rauscher F. J. IIIGenes Dev. 10:2067-2078 (1996) Moosmann P. R., Georgiev O., le Douarin B., Bourquin J. -P., Schaffner W. Nucleic Acids Res. 24:4859-4867 (1996) Agata Y., Matsuda E., Shimizu A. J. Biol. Chem. 274:16412-16422 (1999)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-736

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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