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Western blot analysis in HepG2 and HL-60 cell line lysates (35ug/lane).
TIGD3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hodgkin lymphoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HepG2 and HL-60 cell line lysates (35ug/lane).

TIGD3 Antibody

CATALOG NUMBER: 55-582

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TIGD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-407 amino acids from the C-terminal region of human TIGD3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TIGD3
Additional Names:
Tigger transposable element-derived protein 3, TIGD3
Protein Accession Number:
Q6B0B8
Protein Gi Number:
74762295
Ncbi Gene Id Number:
220359
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the tiggersubfamily of the pogo superfamily of DNA-mediated transposons inhumans. These proteins are related to DNA transposons found infungi and nematodes, and more distantly to the Tc1 and marinertransposases. They are also very similar to the major mammaliancentromere protein B. The exact function of this gene is not known.
Background Reference 1:
Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :
Background Reference 2:
Bonaldo, M.F., et al. Genome Res. 6(9):791-806(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-582

List Size:
400 ul

List Price:

$495.00

Datasheet
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