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Western Blot at 1:2000 dilution Lane 1: EL4 whole cell lysate Lane 2: human fetal thymus lysate Lane 3: human spleen lysate Lane 4: HUT78 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: EL4 whole cell lysate Lane 2: human fetal thymus lysate Lane 3: human spleen lysate Lane 4: HUT78 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

TIGIT Antibody

CATALOG NUMBER: 61-342

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This TIGIT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12-142 amino acids from human TIGIT.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TIGIT
Additional Names:
T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, TIGIT, VSIG9, VSTM3
Protein Accession Number:
Q495A1
Protein Gi Number:
121943253
Ncbi Gene Id Number:
201633
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Background Reference 1:
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Background Reference 2:
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Background Reference 3:
Yu X.,et al.Nat. Immunol. 10:48-57(2009).
Background Reference 4:
Stengel K.F.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404(2012).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-342

List Size:
200 ul

List Price:

$495.00

Datasheet
Shipping Info

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