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A sandwich ELISA was performed using the anti-TIGIT mAb RF16055 (5 μg/ml) as the capture antibody. Biotin-labeled anti-TIGIT mAb RF16058-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.
A sandwich ELISA was performed using the anti-TIGIT mAb RF16055 (5 μg/ml) as the capture antibody. Biotin-labeled anti-TIGIT mAb RF16058-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.

TIGIT ELISA Matched Antibody Pair (Risk Free)

CATALOG NUMBER: RFP-0501

Clonality:
Monoclonal
Tested Applications:
ELISA
Host Species:
Mouse
Species Reactivity:
Human
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
TIGIT antibody was raised against the extracellular domain of human TIGIT
Tested Applications:
ELISA
Set Contents:
Capture: TIGIT Antibody [4A11], Cat. No. RF16055 (100 μg)

Detection: TIGIT Antibody [10B1] (biotin), Cat. No. RF16058-biotin (100 μg)
Purification:
Antibodies are supplied as protein A purified IgG1.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Storage Conditions:
Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).
Background Reference 1:
Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.
Background Reference 2:
Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.
Set Contents:
Capture: TIGIT Antibody [4A11], Cat. No. RF16055 (100 μg)

Detection: TIGIT Antibody [10B1] (biotin), Cat. No. RF16058-biotin (100 μg)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

RFP-0501

List Size:
1 Set

List Price:

$169.00

Out of stock

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