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Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10 μg/mL.
Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20 μg/mL.
Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20 μg/mL.
Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.
Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1 μg/mL.
Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.
Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10 μg/mL.
Recombinant
Trial Size

TIGIT Single Domain Antibody [2C7]

CATALOG NUMBER: SD8829

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P
Host Species:
Llama
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Llama
Species Reactivity:
Human
Immunogen:
TIGIT antibody was raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P
Application Note:
TIGIT antibody can be used for ELISA starting at 0.1 μg/mLimmunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 10μg/mL.
Purification:
TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.  It also contains a myc-tag for detection.
Clonality:
Recombinant Monoclonal
Isotype:
sdAb
physical-state:
Liquid
Buffer:
TIGIT Antibody is supplied in PBS.
Concentration:
1 mg/mL
Storage Conditions:
TIGIT antibody should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TIGIT
Additional Names:
TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Protein Accession Number:
NP_776160
Protein Gi Number:
256600228
Ncbi Gene Id Number:
2016332
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).
Background Reference 1:
Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.
Background Reference 2:
Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

SD8829

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $485.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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