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<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: TIM-1 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4° C (3809) or 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Validation with TIM-1 siRNA Knockdown in HeLa Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 μg of HeLa whole cell lysates per lane.Antibodies: 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: TIM-1 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
KD
Trial Size

TIM-1 Antibody

CATALOG NUMBER: 3811

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (50%), Rat: (43.7%)
Immunogen:
Anti-TIM-1 antibody (3811) was raised against a peptide corresponding to 16 amino acids near the center terminus of human TIM-1.
The immunogen is located within amino acids 230 - 280 of TIM-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 1-8 μg/mL
Antibody validated: Western Blot in human samples. All other applications and species not yet tested
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 3:
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 4:
Cat. No. 1210 - 293 Cell Lysate
Predicted Molecular Weight:
Predicted: 44kD
Observed: 60kD (Post-modification: 4 N-linked glycosylation)
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIM-1 expression profile in human cell lines detected by two independent anti-TIM-1 antibodies that recognize different epitopes, 3809 against N-terminus domain and 3811 against the central domain. TIM-1 proteins are detected in all the tested cell lines except MCF-7 at different expression levels by the two independent antibodies.  

siRNA knockdown validation (Figure 3): Anti-TIM-1 antibody (3811) specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs.

Isoforms:
Human TIM-1 has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD). This antibody detects human isoform b precursor. Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD). Rat TIM-1 has only one isoform identified so far (307aa, 34kD).
Purification:
TIM-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TIM-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HAVCR1
Additional Names:
TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1
Protein Accession Number:
NP_036338
Protein Gi Number:
153085427
Ncbi Gene Id Number:
26762
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
Background Reference 1:
Feigelstock et al. J. Virol. 1998; 72:6621-8
Background Reference 2:
Ichimura et al. J. Biol. Chem. 1998; 273:4135-42
Background Reference 3:
Meyers et al. Mol. Med. 2005; 11:362-9
Background Reference 4:
Mayers et al. Nat. Immunol. 2005;6:455-64.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3811

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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