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<p><strong>Figure 1. Relative Expression Validation of TIM-1 in Mouse Tissues </strong>Loading: 15 μg of mouse tissue lysate of each lane Antibodies: TIM-1 9927, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.<p><strong>TIM-1 antibody (9927) detected high expression levels of TIM-1 in the mouse kidney and testis, while showing very low or no expression in the lung, brain and thymus. This pattern aligns with the RNA expression data for these tissues in human, as reported by Human Protein Atlas. </strong>
<p><strong>Figure 2. WB Validation in Rat Liver </strong>Loading: 15 μg of rat liver lysate Antibodies: TIM-1 9927, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 1. Relative Expression Validation of TIM-1 in Mouse Tissues Loading: 15 μg of mouse tissue lysate of each lane Antibodies: TIM-1 9927, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

TIM-1 antibody (9927) detected high expression levels of TIM-1 in the mouse kidney and testis, while showing very low or no expression in the lung, brain and thymus. This pattern aligns with the RNA expression data for these tissues in human, as reported by Human Protein Atlas.

New
Trial Size

TIM-1 Antibody

CATALOG NUMBER: 9927

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Human: (63%).
Immunogen:
Anti-TIM-1 antibody (9927) was raised against a peptide corresponding to 16 amino acids near the amino terminus of mouse TIM-1.

The immunogen is located within amino acids 50 - 100 of TIM-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TIM-1 antibody can be used for the detection of TIM-1 by Western blot at 1-4 μg/mL.

Antibody validated: Western Blot in mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1405 – Mouse Kidney lysate
Positive Control 2:
Cat. No. 1416 – Mouse Testis lysate
Positive Control 3:
Cat. No. 1464 – Rat Liver lysate
Validation:

Relative Expression Validation (Figure 1): Anti-TIM-1 antibodies (9927) specificity was further verified by TIM-1 relative expression. TIM-1 antibody (9927) detected high expression levels of TIM-1 in the mouse kidney and testis, while showing very low or no expression in the lung, brain and thymus. This pattern aligns with the RNA expression data for these tissues in human, as reported by Human Protein Atlas. (https://www.proteinatlas.org/ENSG00000113249-HAVCR1/tissue).

Isoforms:
Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD). Rat TIM-1 has only one isoform identified so far (307aa, 34kD).
Purification:
TIM-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TIM-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HAVCR1
Additional Names:
TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1
Protein Accession Number:
Q5QNS5.2
Protein Gi Number:
73919878
Ncbi Gene Id Number:
171283
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
Background Reference 1:
Feigelstock et al. J. Virol. 1998; 72:6621-8
Background Reference 2:
Ichimura et al. J. Biol. Chem. 1998; 273:4135-42
Background Reference 3:
Meyers et al. Mol. Med. 2005; 11:362-9
Background Reference 4:
Mayers et al. Nat. Immunol. 2005;6:455-64.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9927

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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