Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of TIM-3 in Jurkat cell lysate with TIM-3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TIM-3 in human spleen tissue with TIM-3 antibody at 2 μg/ml.
Immunofluorescence of TIM-3 in human spleen tissue with TIM-3 antibody at 20 μg/ml.<br><br>Red: TIM-3 Antibody (8659) <br> Blue: DAPI staining
Western blot analysis of TIM-3 in Jurkat cell lysate with TIM-3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TIM-3 Antibody

CATALOG NUMBER: 8659

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
TIM-3 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-3.
The immunogen is located within amino acids 60 - 110 of TIM-3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TIM-3 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two antibodies are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
Predicted: 33 kDa
Observed: 37 kDa
Purification:
TIM-3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TIM-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HAVCR2
Additional Names:
TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM-3, CD366, KIM-3, TIMD3, TIMD-3
Protein Accession Number:
NP_116171
Protein Gi Number:
84868
Ncbi Gene Id Number:
84868
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM-3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
Background Reference 1:
Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41.
Background Reference 2:
Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187–94.
Background Reference 3:
Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501–10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8659

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369