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Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 μg/mL.
Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 μg/mL.
Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 μg/ml.
Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 μg/ml.<br><br>Green: TIM-4 Antibody (3815) <br> Blue: DAPI staining
Immunohistochemistry of TIM-4 in mouse liver tissue with TIM-4 antibody at 2 μg/ml.
Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Green Product
Trial Size

TIM-4 Antibody

CATALOG NUMBER: 3815

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TIM-4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-4.
The immunogen is located within amino acids 60 - 110 of TIM-4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of TIM-4 are known to exist; this antibody will detect all four isoforms.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 1283 - RAW 264.7 Cell Lysate
Positive Control 3:
Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight:
Observed: 45 kDa
Purification:
TIM-4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
TIM-4 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TIM-4 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TIMD4
Additional Names:
TIM-4 Antibody: TIM4, SMUCKLER, TIM4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, TIMD-4
Protein Accession Number:
NP_612388
Protein Gi Number:
226529863
Ncbi Gene Id Number:
91937
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TIM-4 Antibody: The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.
Background Reference 1:
Meyers JH, Sabatos CA, Chakravarti S, et al. The TIM family regulates autoimmune and allergic diseases. Trends Mol. Med. 2005; 11:362-9.
Background Reference 2:
Meyers JH, Chakravarti S, Schlesinger D, et al. TIM-4 is the ligand for TIM-1, and the TIM-1-TIM4 interaction regulates T cell proliferation. Nat. Immunol. 2005; 6:455-64.
Background Reference 3:
Kuchroo VK, Umetsu DT, DeKruyff RH, et al. The TIM gene family: emerging roles in immunity and disease. Nat. Rev. Immunol. 2003; 3:454-62.
Background Reference 4:
Shakhov AN, Rybtsov S, Tumanov AV, et al. SMUCKLER/TIM4 is a distinct member of TIM family expressed by stromal cells of secondary lymphoid tissues and associated with lymphotoxin signaling.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3815

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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