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46-496 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-496 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TIM-50 Antibody

CATALOG NUMBER: 46-496

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CDVRTVLEHYALEDD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 39kDa in Human Heart lysate (Calculated MW of 50.5kDa according to NP_001001563.1) . The observed molecular weight corresponds to earlier finding in literature with different antibodies (Guo et al, J Bio Chem 2004 Jun 4;279:24813-25; PMID: 15044455). Recommended concentration: 0.5-1.5ug/ml.
Specificity:
This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TIMM50
Additional Names:
TIMM50, TIM50L, translocase of inner mitochondrial membrane 50 homolog (yeast), Tim50-like protein, homolog of yeast Tim50, translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae), MGC102733, TIM50
Protein Accession Number:
NP_001001563.1
Protein Gi Number:
48526509
Ncbi Gene Id Number:
92609
Background Reference 1:
Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber SA, Fernandes-Alnemri T, Alnemri ES. Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. J Biol Chem. 2004 Jun 4;279(23):24813-25. Epub 2004 Mar 25.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-496

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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