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IHC testing of FFPE human placenta with TIMP2 antibody (clone SPM356). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human placenta with TIMP2 antibody (clone SPM356). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

TIMP2 Antibody [SPM356]

CATALOG NUMBER: 33-734

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
An amino acid sequence from the N-terminal region of the human protein was used as the immunogen for this TIMP2 antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
WB: 0.5-1 ug/ml
IF: 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
TIMP2
Additional Names:
TIMP2, Metalloproteinase inhibitor 2, TIMP-2, CSC-21K
Ncbi Gene Id Number:
7077
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2). It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP9 or gelatinase B). TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-734

List Size:
100 ug

List Price:

$626.00

Datasheet
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