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Western blot analysis of TIMP3 Antibody in HepG2 cell line lysates (35ug/lane)
Western blot analysis of TIMP3 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TIMP3 Antibody in HepG2 cell line lysates (35ug/lane)

TIMP3 Antibody

CATALOG NUMBER: 63-791

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Rabbit, Rat
Immunogen:
This TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-139 amino acids from the Central region of human TIMP3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TIMP3
Additional Names:
Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3
Protein Accession Number:
P35625
Protein Gi Number:
730948
Ncbi Gene Id Number:
7078
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TIMP3 is one of inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM).
Background Reference 1:
Jacobson,S.G., et.al., Nat. Genet. 11 (1), 27-32 (1995) Wick,M., et.al., Biochem. J. 311 (PT 2), 549-554 (1995)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-791

List Size:
400 ul

List Price:

$495.00

Datasheet
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