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Western blot analysis of extracts of various cell lines, using TKFC antibody (16-040).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using TKFC antibody (16-040).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

TKFC Antibody

CATALOG NUMBER: 16-040

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 226-575 of human TKFC (NP_056348.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:2000
Positive Control 1:
293T
Positive Control 2:
LO2
Positive Control 3:
HeLa
Positive Control 4:
Mouse heart
Positive Control 5:
Rat testis
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TKFC
Additional Names:
DAK, NET45, triokinase/FMN cyclase, ATP-dependent dihydroxyacetone kinase, DHA kinase, Dha kinase/FMN cyclase, FAD-AMP lyase cyclic FMN forming, FAD-AMP lyase cyclizing, bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing), dihydroxyacetone kinase 2 homolog, glycerone kinase, testis tissue sperm-binding protein Li 84P
Ncbi Gene Id Number:
26007
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-040

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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