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To detect hTL-1A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human TL-1A (38-162) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTL-1A.
To detect hTL-1A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human TL-1A (38-162) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTL-1A.

TL-1A Antibody (biotin)

CATALOG NUMBER: 38-163

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTL-1A. Human TL-1A specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hTL-1A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human TL-1A as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTL-1A.
Western Blot:
To detect hTL-1A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTL-1A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
TL-1A antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
TNFSF15
Additional Names:
TL1, TL1A, VEGI, VEGI192A, TL1, Tumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1,
Protein Accession Number:
O95150
Protein Gi Number:
189047102
Ncbi Gene Id Number:
9966
User Note:
Centrifuge vial prior to opening.
Background:
TL1A is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a TNF like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator. TL1A is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL 1 alpha. TL1A can activate NF kappa B and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells and may function as an angiogenesis inhibitor. TL1A is a longer variant of TL1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-163

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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