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Western blot analysis of TLE1 Antibody in Jurkat cell line lysates (35ug/lane)
Western blot analysis of TLE1 Antibody in Jurkat cell line lysates (35ug/lane)

TLE1 Antibody

CATALOG NUMBER: 64-027

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TLE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the Central region of human TLE1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TLE1
Additional Names:
Transducin-like enhancer protein 1, E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1, TLE1
Protein Accession Number:
Q04724
Protein Gi Number:
29840816
Ncbi Gene Id Number:
7088
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Background Reference 1:
Ali, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 107(9):4165-4169(2010)
Background Reference 2:
Jagdis, A., et al. Am. J. Surg. Pathol. 33(12):1743-1751(2009)
Background Reference 3:
Kosemehmetoglu, K., et al. Mod. Pathol. 22(7):872-878(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-027

List Size:
400 ul

List Price:

$495.00

Datasheet
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