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42-117 (1ug/ml) staining of Human Peripheral Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-117 (1ug/ml) staining of Human Peripheral Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TLN1 Antibody

CATALOG NUMBER: 42-117

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QEALHTQMLTAVQE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:A >250kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 270kDa according to NP_006280.3). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 110kDa. This band was successfully blocked by incubation with the immunizing peptide.
Specificity:
This antibody is not expected to cross-react with the highly similar protein Talin 2.
Positive Control 1:
Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TLN1
Additional Names:
TLN1, talin 1, ILWEQ, KIAA1027, TLN
Protein Accession Number:
NP_006280.3
Protein Gi Number:
223029410
Ncbi Gene Id Number:
7094
Background Reference 1:
Manevich E, Grabovsky V, Feigelson SW, Alon R, Talin 1 and paxillin facilitate distinct steps in rapid VLA-4-mediated adhesion strengthening to vascular cell adhesion molecule 1. The Journal of biological chemistry 2007 Aug 282 (35): 25338-48.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-117

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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