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Western blot analysis of TLR4 in human small intestine lysate with TLR4 antibody at (A) 2 and (B) 4 μg/mL.
Immunocytochemistry of TLR4 in K562 cells with TLR4 antibody at 2 μg/mL.
Immunohistochemistry of TLR4 in human spleen tissue with TLR4 antibody at 2.5 μg/ml.
Immunofluorescence of TLR4 in human spleen tissue with TLR4 antibody at 20 μg/ml.<br><br>Green: TLR4 Antibody (3141) <br> Blue: DAPI staining
Western blot analysis of TLR4 in human small intestine lysate with TLR4 antibody at (A) 2 and (B) 4 μg/mL.
Trial Size

TLR4 Antibody

CATALOG NUMBER: 3141

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (80%), Horse: (73%), Rat: (71%), Pig: (73%)
Immunogen:
TLR4 antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of human TLR4.
The immunogen is located within amino acids 30 - 80 of TLR4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
TLR4 antibody can be used for detection of TLR4 by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 3:
Cat. No. 17-004 - K-562 Cell Slide
Predicted Molecular Weight:
Predicted: 92 kDa
Observed: 94 kDa
Purification:
TLR4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TLR4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TLR4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TLR4
Additional Names:
TLR4 Antibody: TOLL, CD284, TLR-4, ARMD10, Toll-like receptor 4, hToll
Protein Accession Number:
NP_612564
Protein Gi Number:
19924149
Ncbi Gene Id Number:
7099
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TLR4 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.
Background Reference 1:
Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
Background Reference 2:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 3:
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Background Reference 4:
O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3141

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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