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Western blot analysis of TLR7 in Daudi cell lysates with TLR7 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunocytochemistry staining of Daudi cells using TLR7 antibody at 2 μg/mL.
Western blot analysis of TLR7 in Daudi cell lysates with TLR7 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

TLR7 Antibody

CATALOG NUMBER: 3269

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TLR7 antibody was raised against a peptide corresponding to 14 amino acids near the center of mouse TLR7.
The immunogen is located within amino acids 420 - 470 of TLR7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
TLR7 antibody can be used for detection of TLR7 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight:
Predicted: 116 kDa
Observed: 135 kDa
Purification:
TLR7 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TLR7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TLR7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Tlr7
Additional Names:
TLR7 Antibody: Toll-like receptor 7
Protein Accession Number:
NP_573474
Protein Gi Number:
18875360
Ncbi Gene Id Number:
170743
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TLR7 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus. Activation of TLR7 by VSV results in stimulation of the immune response including IFNalpha secretion, suggesting the importance of TLR7 in virus recognition.
Background Reference 1:
Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
Background Reference 2:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 3:
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Background Reference 4:
O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3269

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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